Qiagen maxiprep kit manual

Compactprep plasmid kits require use of a vacuum device e. They are not to be used for human diagnostic or drug purposes or to be administered to humans unless expressly cleared for that purpose by the food. Processing, manual centrifugation, manual centrifugation, manual centrifugation, manual centrifugation, manual. Apply the supernatant from centrifugation to the qiagen tip 20 and allow it to enter the resin by gravity flow.

Qiagen plasmid plus maxi kit vs zymopure plasmid maxiprep kit 25. The patented resin provides excellent capacity with fast flow rates, high resolution, high yield, and efficient endotoxin removal. Product information thermo scientific genejet plasmid. The resin bed will retain some buffer, and will not readily dry out, of the tips can be left unattended. The kit recovers up to 20 g of high copy plasmid dna per isolation procedure. Instruction manual 1 1 note integrity of kit components is guaranteed for up to one year from date of purchase. The maxiprep kit protocol will typically yield 500850 g plasmid dna from 100200 ml bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients. For isolation of genomic, mitochondrial, bacterial, parasite or viral dna. Mix gently by inverting the tube 46 times and incubate at room temperature for exactly 5 min. Full plasmid dna extraction completed using a qiagen maxiprep kit. This protocol is designed for purification of total dna from grampositive bacteria.

Qiaprep miniprep kits use silicagelmembrane technology to eliminate the cumbersome steps associated with loose resins or slurries. For transfection of sensitive cell lines endotoxins are a major pr. Pureyield plasmid maxiprep system technical manual. The purchaser must determine the suitability of the product for its particular use.

Up to 24 samples can be processed in parallel in less than 60 minutes using a vacuum manifold e. In accordance with qiagens isocertified quality management system, each lot of the rneasy mini kit, rneasy protect mini kit, and rneasy plant mini kit is tested against predetermined specifications to ensure consistent product quality. Genelute hp endotoxinfree plasmid kits include a filter syringe for clearing lysates and binding columns that are used with a vacuum format. Hispeed plasmid purification handbook harvard university.

Isolate ii plasmid mini kit is designed for the rapid and efficient isolation of highly pure and maximal yields of plasmid dna from bacterial cultures using silicamembrane spin column technology. For purification of up to 10 mg transfectiongrade plasmid or cosmid dna. Qiagen plasmid kits should be stored at room temperature 1525c. They can be stored for at least 2 years without showing any reduction in performance, capacity, or quality of separation. This procedure makes these kits the fastest largescale prep available 20 minutes for maxi and midi preps. Qiagen plasmid purification handbook kirschner lab. For purification of ultrapure, transfection grade plasmid dna.

The protocol for hispeed midi and maxi kits is optimized for use with. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the zymopure ii plasmi. Plasmid midi kit and 50250 g for the hispeed plasmid maxi kit. In as little as 4 hours up to 500g of supercoiled dna is available for use.

Genelute hp plasmid dna midiprep kit 25 purifications. After adding rnase a, buffer p1 should be stored at 28c and is stable for 6. Qiaprep miniprep handbook washington state university. The qiagen plasmid plus kits provide the most convenient method for largescale plasmid preparation. Features and benefits from harvested bacterial culture to pure plasmid dna in 30 minutes or less up to 350. Jul 20, 2015 this tutorial explains how to carry out a miniprep using the qiaprep spin miniprep kit provided by qiagen. It should be noted that this alteration does not work with the qiafilter maxi kit, as the syringe is not designed to hold the increased volume.

The kit passes qc requirements if the purified plasmid dna has an a 260 a. Revised 1109 tm280 technical manual pureyield plasmid maxiprep system instruc ons for use of products a2391, a2392 and a2393 note. Na0300 genelute hp plasmid maxiprep kit 10 preps na0310 genelute hp plasmid maxiprep kit 25 preps. The qiagen plasmid kits uses gravityflow qiagen anionexchange tips for efficient purification of plasmid dna. Qiagen plasmid maxi kit, 100 preps, 100 to 500ml culture volume, transfectiongrade plasmid or cosmid dna purification, anionexchange technology, 500g yield, 160 min. Using the etf maxi kit from qiagen allows fast and efficient isolation of plasmid dna suitable for transfection and cloning. Isolate ii plasmid mini kit is designed for the rapid and efficient isolation of highly pure and maximal yields of plasmid dna from bacterial cultures. Zymopure ii plasmid kits are the fastest and simplest plasmid kits available to efficiently isolate transfectiongrade plasmid dna from e. Qiagen plasmid purification handbook 082003 3 contents kit contents 5 storage 8 technical assistance 8 product warranty and satisfaction guarantee 8 product use limitations 9 safety information 9 introduction 10 comparison of qiagen largescale plasmid purification options 10 principle and procedure. Our technical service departments are staffed by experienced scientists with extensive practical and theoretical expertise in molecular biology and the use of qiagen prod ucts. Highlights efficienthigh yields of up to 20 g of high quality plasmid dna fastpr. Part of the zymopure plasmid kits collection, the zymopure ii plasmid maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 1. Thermo scientific genejet plasmid maxiprep kit is qualified by isolating high copy number plasmid dna from 250 ml of overnight e.

The endofree maxiprep kit from qiagen or the genejet maxiprep kit from thermo fisher were used for maxi preparations. The purelink hipure plasmid filter kits include hipure filter columns, which combine filtration for clarification of the bacterial lysate wit. The troubleshooting guide supports you with molecular biology applications only. Thermo scientific genejet plasmid maxiprep kit, sufficient for 25 preps, utilizes silicabased membrane technology in the form of convenient spin column. Qiagen plasmid maxi kit 100, 12165 from qiagen labsave. Multi sample processing is often required to complete both plasmid isolation and subsequent downstream experimentation. Thermo scientific genejet plasmid midiprep kit is qualified by isolating high copy plasmid dna from 50 ml of overnight e. K210026 and k210027, to rapidly precipitate eluted dna with isopropanol, without using a centrifuge. Thermo scientific genejet plasmid miniprep kit utilizes an exclusive silicabased membrane technology in the form of a convenient spin column. Qiagen plasmid mini, midi, maxi, mega, and giga kits. Under these conditions, the components are stable for 6 months without showing any reduction in performance and quality, unless otherwise indicated on the. Qiagen plasmid mini kit, 100 preps, 3 to 10ml culture volume, 80 min. Hipure plasmid fp filter and precipitator maxiprep kit cat.

From these plasmid kits, the eluted plasmid dna is ready for immediate use, without any alcohol precipitation. Plasmid dna was purified from bacterial cultures using nucleobond xtra midi, nucleobond xtra maxi, and corresponding midiprep and maxiprep kits from qiagen following the protocols for each kit in each case, the maximum input volume specified by the corresponding kit protocol was processed. Thermo fisher scientific this kit is good for acquiring high purity plasmid dna. Please consult the material safety data sheet msds for information regarding hazards and safe handling. The maxiprep kit protocol will typically yield 500850 g plasmid dna from 100200 ml bacterial. Qiaprep spin midimaxiprep kit protocol before staring make sure that rnase a has been added to p1 buffer.

The zymopure ii plasmid maxiprep kit features a simple spincolumn based method for the purification of up to 1. Following is the miniprepqiagen kit protocol in biocoder, a highlevel programming language for expressing biology protocols. Compactprep plasmid kits provide fast spin columnbased largescale plasmid purification. Qiaprep spin miniprep kit protocol using a microcentrifuge this protocol is designed for purification of up to 20 g of highcopy plasmid dna from 15ml overnight cultures of liin lb luriabertani medium. Centrifugation tubes or vessels with suitable capacity for the volumes specified in the appropriate protocol. Plasmid or cosmid dna purification using qiagen plasmid midi and. Under these conditions, if no expiration date is mentioned on the kit label, qiaprep miniprep kits can be stored for up to 12 months without showing any reduction in.

Zymopure ii plasmid kits get free sample now zymo research. Certificate of analysis thermo scientific genejet endofree plasmid maxiprep kit is qualified by isolating high copy number plasmid dna from 350 ml of overnight e. Sigmas genelute hp plasmid maxiprep kits offer a simple, rapid, and costeffective method for isolating plasmid dna from recombinant e. Qiagen plasmid purification handbook sierra bermeja. Genelute hp plasmid midiprep kit has been used to isolate and purify plasmids. Chargeswitch pro filter plasmid midiprep and maxiprep kits. Full qiagen maxiprep plasmid dna extraction with steps. Endofree plasmid maxiprep kit syringe format notice. More information about biocoder can be found on my home page. Total rna and mirna can also be copurified from formalinfixed, paraffinembedded. Thermo scientific genejet endofree plasmid maxiprep kit is a fast, convenient, and efficient system for the large scale purification of endotoxinfree plasmid dna. Qiagen plasmid purification handbook 082003 9 product use limitations qiagen, qiafilter, and endofree plasmid kits are developed, designed and sold for research purposes only.

After adding rnase a, buffer p1 should be stored at 28c. Midipreps for plasmid dna purificationnucleobond xtra midi. Filmed using an iphone xsmax and inbuilt imovie features. Each prep recovers up to 750 g of high copy plasmid dna that can be used in a wide variety of molecular biology procedures, such as restriction en.

Invitrogen plasmid maxiprep kits offer a range options to eliminate the challenges raised by this level of workflow processing. For the qiagen plasmid mini kit protocol microcentrifuge 1. The purelink hipure plasmid maxiprep kit is designed to isolate transfectiongrade plasmid dna from e. Qiagen tips should be stored dry and at room temperature 1525c.

The design and unique binding chemistry of the qiagen plasmid plus columns allow a simple bindwashelute procedure based on a novel chemistry. Plasmid maxiprep preparation is a basic technique performed in most molecular biology laboratories. Equilibrate a qiagen tip 20 by applying 1 ml buffer qbt, and allow the column to empty completely by gravity flow. What you see here is the autogenerated text ouput of the protocol that was coded up in biocoder see source code. The purelink hipure plasmid maxiprep kit is designed to isolate. Qiagen plasmid mini kit 100 from qiagen selectscience. As research moves from dna sequencing to analysis of gene function, the need has increased for rapid methods for isolating large quantities of highquality plasmid dna. Reagents are routinely tested on a lottolot basis to ensure they provide maximal performance and reliability. This support tool is not for products for the diagnosis, prevention, or treatment of a disease. Qiagen plasmid plus maxi kit vs zymopure plasmid maxiprep.

Brief considerations for plasmidcosmid purification procedures. The ultrafast purification protocol, based on the remarkable selectivity of patented qiagen resin, allows the isolation of ultrapure, supercoiled plasmid dna. The genelute hp endotoxinfree plasmid maxiprep kit offers a novel and efficient method of isolating endotoxinfree plasmid dna in 40 minutes. A resource portal for protocols in bioscience including molecular biology, cell biology, immunology, plant biology, developmental biology, neuroscience, bioinformatics, genetics. Midipreps for plasmid dna purificationnucleobond xtra. Using this module saves time and reduces the risk of losing the dna pellet during supernatant removal. The purelink hipure plasmid filter maxiprep kit is designed to isolate plasmid dna of the highest purity from 100 to 500 ml of an overnight bacterial culture.

Should any product fail to perform satisfactorily due to any reason other. Technical manual pureyield plasmid maxiprep system instruc ons for use of products a2391, a2392 and a2393 note. The purelink hipure plasmid dna purification kits use a patented anionexchange resin to purify plasmid dna to a level equivalent to two passes through cscl gradients. The eluted plasmid dna is endozero and ready for immediate use in the most sensitive applications. When transferred into filter syringelet stand the lysate at room, temperature for 1 to 2 min until the white precipitates float to the top, then press the plunger. Endotoxins are major contaminants found in plasmid dna preparations. In accordance with qiagen s isocertified quality management system, each lot of the rneasy mini kit, rneasy protect mini kit, and rneasy plant mini kit is tested against predetermined specifications to ensure consistent product quality. Plasmid dna maxiprep kits plasmid dna midiprep kits plasmid dna miniprep kits. Add 10 ml of buffer p3, mix gently, incubate on ice for 1520 min. Yield and purity measurements of plasmid dna isolated from pureyield and qiagen maxiprep. The protocol describes the preliminary harvesting of bacteria and incubation with lysozyme to lyse their cell walls before dna purification. Qiagen, qiafilter, hispeed, and endofree plasmid kits, provide optimal.

Qiagen plasmid purification handbook harvard university. For highthroughput purification in a 96well format, the mirneasy 96 kit is available. May 24, 2010 megaprep plasmid dna extraction kit is used when the starting e. For purification of up to 10 mg endotoxinfree advanced transfectiongrade plasmid or cosmid dna. Plasmid or cosmid dna purification using qiagen plasmid midi and maxi. Purelink hipure plasmid maxiprep kit thermo fisher scientific. Other extraction and purification plasmid dna kits are available from varying manufacturers, named by size of bacterial culture, includes gigaprep, midiprep, and. Plasmid dna maxiprep kits thermo fisher scientific ca. If grown too long, i have been told that the pec9 plasmid will loose.